Following this in cubation the wells were washed as above and 100 [micro]L of goat anti-dog IgG (H & L) peroxidase conjugate (Kirkegaard and Perry, Gaithersburg, MD, USA) was added to each well and incubated for 30 min at 37[degrees]C.
Housing in cubation and grow-on space, the facility will provide as pring board for entrepreneurs and provide a welcome boost to the local economy with the potential of creating up to 500 jobs.