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Next, 1 [micro]L of the extracted DNA was used in PCR with primers Dino1662F and 28SR2 to amplify a ~1 900 base pair region of the rRNA gene cistron covering 3' end region of the SSU, the entire ITS, and partial 5' region of the LSU (3'end-SSU-ITS1-5.8S-ITS2-LSU) (Qiu, Huang, Liu, Zhang, & Lin, 2013).
Polymerase chain reaction (PCR) was initially used to amplify part of the mitochondrial cytochrome oxidase c subunit 1 gene (COI) and the complete internal transcribed spacer 2 gene (ITS2) of the nuclear ribosomal cistron (rRNA).
Additionally, reinitiation of terminating ribosomes will typically not occur on the downstream cistron; thus, the translation of the main coding sequence is inhibited in these conditions [24].
Complete sequences of the ITS1-5.8S-ITS2 region of the rDNA cistron (977bp) were obtained for 16 samples of nematodes.
The ITS region of ribosomal DNA (rDNA) cistron has been primarily sequenced in fungi for the use of diversity documentation intra- and interspecies level classification phylogenetic analyses and environmental sampling; because of their high degree of variation than the 5S 5.8S 18S small subunits and 25S28S large subunit in rDNA (O'Brien et al.
MEM was purchased from Hi Media Laboratories and Fetal bovine serum (FBS) was purchased from Cistron laboratories.
Variation in the ribosomal RNA cistron among host-adapted races of ah aphid (Schizaphis graminum).